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August 1993, Volume 43, Issue 8

Original Article

Significance, Isolation, Identification and Sensitivity of Branhaemella (Moraxella) Catarrhalis

Salim Hafiz  ( Zafar Research and Diagnostic centre, 7th Floor, Rimpa Plaza, M.A. Jinnah Road, Karachi. )
Tahira Hafiz  ( Zafar Research and Diagnostic centre, 7th Floor, Rimpa Plaza, M.A. Jinnah Road, Karachi. )
Iffat Yazdani  ( Liaquat National Hospital, Karachi. )


In a study lasting over two years the frequency and sensitivity of moraxella catarrhalis causing respiratory tract infections were studied. Sputum samples from patients with lower respiratory tract infections were screened for moraxella catarrhalis. The organisms isolated ideniffed and their sensitivity determined by simple methods which are practicable. The study shows that 22.4% of the infections were due to moraxella catarrhalis and 98% of the isolates were sensitive to amoxicillin-culvanalic acid (augmentin). The paper signifies the importance of reporting moraxella catarrhalis and its treatment (JPMA 43: 153, 1993).


The organism was first reported by Frosch and Kolle in 1896 and named as micrococcus catarrhalis meaning a small coccus down flowing catarrh. Ghon and Pfeiffer studied and stated that in sputum it occurred in pairs, tetrads or occasionally in small groups and produced colonies resembling those of streptococcus pyogenes. Elser and Huntoon described two types of colonies being produced by the organism1. The organism was renamed as neisseria catarrhalis as it rcsembled the neisseria group2. Its name was changed to branhaemella catar­rhalis as it differs from neisseria in its DNA base content and fatty acid composition. Now the organism is renamed as moraxella catarrhalis3. The organism has been recognised as a cause of human infection since the early 1900’s but only in 1982 it received a clear appreciation due to the extent of disease in which it produces. It causes a wide variety of human infections, ranging from systemic life threatening dis­eases such as endocarditis and meningitis4 to acute, localized infections including acute otitis media, sinusitis and bronchopulmonary infections5-7. In certain condi­tions M. catarrhalis is the second or third most common cause of bacterial infections of the lower respiratory tract4. It is also associated with frank pneumonia and superinfections in patients with active tuberculosis3. Since there is no record of the actual extent of M. catarrhalis infection in Pakistani population a prospec­tive study was planned to study the frequency and sensitivity of pulmonary infection due to M. catarrhaiis.

Patients and Methods

Patients with the evidence of chest infections and symptoms of chronic obstructive airway disease were selected for bacteriological screening. Two hundred and fifty sputa and 150 bronchial aspirates were collected from out-patients and in- patients attending different hospitals and coming to chest physicians of Karachi over the period of one year. The sputa were screened for acceptability based on the cellular criteria8 and grams stain was performed which is very helpful in the diagnosis of M. catarrhalis broncho-pulmonary infections. All sputa were inoculated onto blood agar and chocolate agar plates and incubated at 35-36°C in a candle jar for 24-48 hours. M. catarrhalis produced small round, entire whitish grey colonies which could be pushed across the plates with a bacteriological loop, and the colonies were oxidase positive strongly suggestive of M. catarrhalis. The isolates were grams stained, oxidase tested, car­bohydrate fermentation carried out and tested for deoxyribonuclease activity9. The organisms were oxidase and catalase positive fail to ferment glucose, maltose, sucrose and lactose or to grow on nutrient agar at room temperature. Sensitivity tests were carried out against penicillin, a.mpicillin, augmentin, cotrimoxazole, erythromycin, tetracycline and cepharidine using the stokes disc diffusion method10 and scnsitivity recorded11.


A total of 250 sputa and 150 bronchial aspirates were obtained and processed for the presence of M. catarrhalis mixed infections in which streptococcus pneumoniae, haemophilus influenzae and group A beta haemolytic streptococci were found alongwith M. catar­rhalis were excluded and only cases where M. catarrhalis was seen as the only pathogen were included in the study.

Table I shows the frequency of infection due to M. catarrhalis seen in 400 cases. The overall infection rate was found 21.5%. Twenty-three percent of bronchial aspirates yielded the pathogen while sputum resulted in 20.8% cases indicating that both samples are suitable for the isolation of this pathogen.

Table II gives the sensitivity pattern of 86 isolates to the antibiotics tested. Most (96.5%) isolates were found to be sensitive to amoxycillin clavulanate (augmentin) and 90.6% were sensitive to erythromycin. Fifty-three percent of the isolates were resistant to penicillin, ampicillin/amoxil and 49% were resistant to cotrimoxazole.


Moraxella (branhaemella) catarrhalis is now regarded as pathogen and is being reported and treated in the western world12. The organism is encountered in our population and causes respiratory tract infections. The present data shows that the organisms can be recovered from sputum and bronchial aspirates, hence, for culture bronchial aspirates or sputum are both suitable. The pathogen can be grown easily and the methods of identification are within the reach of an average clinical laboratory. There is evidence of beta lactamase production by M. catarrhalis which is an alarming situation. Most of the isolates are sensitive to amoxycillin-clavulanate (augmentin) and erythromycin and seems to be the antibiotic of choice for treating infections due to M. catarrhalis. Finally, M. catarrhalis has emerged as an important clinical pathogen in our part of the world and probably causes diseases more frequently than realized, hence it should be looked for isolated, reported and treated.


1. Ghon, A. and Pfeiffer, H. Thc principles of bacteriology and immunity. Edited by W.W.C., Topley and G.S. Wilson, 2nd ed. Published by Edward Arnold and Co., U.K., 1926, pp. 422-423.
2. Catlin, B.W. Transfer of organism named neisseria catarrhslis to branhamella genus. Nov. IntJ.Syst. Bacteriol., 1970;20:155-58.
3. Hamedani, P., Haliz, S., Ali, J., Memon, R. et al. Respiratory symptoms due to branhamella catarrhslis and other neisseria species. Infections-response to erythromycin therapy. Clin.Ther., 1989;11:633-39.
4. Doern, CV. Branhamellscatsrrhalis - an emerging human pathogen. Diagn. Microbial. Infect. Dis., 1986;4:191-201.
5. Glebink. G.S. The microbiology of otitit media. Pediatr.Infect. Ohs. J., 1989;8518-20.
6. 1-lager, H., Varghese, A., Alvarez, S. and Bert, S.L. Brsnhsmells cstarrhalis respiratory infection. Rev. Infect. Dis., 1987;9:1140-49.
7. Van Hare, G.F. and Shurin, PA. The incressingirnportance of branhsmella cstarrhslis in respiratory infections. Pediatr. Infect. Dis. .1., 1987;6:92-94.
8. Aitken, 3M. and Thomley, P.E. Isolation of branhamella catarrhalis from sputum and tracheal aspirate. J.Clin.Microbiol., 1983;18:1262-63.
9. Cowan, S.P. Cowan and Steel’s Manual for the identification of medical bacteria. 2nd ed., London, Cambridge University Press, 1985, pp. 80-84.
10. Stokes, ff2. and Wsterworth, P.M. Antibiotic sensitivity teat by diffusion method.). Assoc. Cfln. PathoL Board, 1972;55:1-i2.
11. Luman, LR., Wilson, W., Wallace, R.J. and Nash, D.R. Disk diffusion susceptibility of brsnhsmella cstarrhalis and relationship of beta lactam zone size tpo beta lactamase production. Antimicrob. Agents chemotherapy, 1986;30:774-76.
12. Sarubbl, F.A., Myers.J.W. and Jennifer., J. Rcspiratoryinfections caused bybranhamella catarrhalis, Am.J.Med., 1990;88 (suppl):54A-145.

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